Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing
نویسندگان
چکیده
منابع مشابه
Statistical medium optimization of an alkaline protease from Pseudomonas aeruginosa MTCC 10501, its characterization and application in leather processing.
Proteases are shown to have greener mode of application in leather processing for dehairing of goat skins and cow hides. Production of protease by submerged fermentation with potent activity is reported using a new isolate P. aeruginosa MTCC 10501. The production parameters were optimized by statistical methods such as Plackett-Burman and response surface methodology. The optimized production m...
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An intracellular protease was extracted and purified from Pseudomonas aeruginosa by ion-exchange chromatography on DEAE-cellulose followed by CM–cellulose and rechromatography on DEAE-cellulose. The purified protease was found to be homogeneous as judged by polyacrylamide disc gel electrophoresis (PAGE). The molecular mass of the protease as determined by gel filtration on G-150 was about 48,00...
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A protease isolated from Pseudomonas aeruginosa PD100 could act in the presence of SDS and Tween 80. This protease could be useful for degradation of protein in the presence of solvent, dehairing of cow skin and degradation of natural proteins. The immobilized protease showed 15-20% increases in temperature stability and the entrapped enzyme retained 83% of its initial activity after six cycles...
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A highly sensitive and specific radioimmunoassay has been developed for Pseudomonas aeruginosa alkaline protease. Production of alkaline protease was found to be strain variable and medium dependent.
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Mucoid strains of Pseudomonas aeruginosa that overproduce the exopolysaccharide alginate are a frequent cause of chronic respiratory infections in cystic fibrosis (CF) patients. The overproduction of alginate by these strains is often caused by mutations within mucA of the algU mucABCD gene cluster. This gene cluster encodes an extreme stress response system composed of the ECF alternative sigm...
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ژورنال
عنوان ژورنال: Journal of Genetic Engineering and Biotechnology
سال: 2021
ISSN: 2090-5920
DOI: 10.1186/s43141-021-00149-8